Aquat. Living Resour.
Volume 35, 2022
Special Issue - NORA 4
|Number of page(s)||13|
|Published online||20 May 2022|
Molecular identification, life cycle characterization, and hatchery seed production of dwarf oysters from the Ebro Delta (Spain)
IRTA-Sant Carles de la Ràpita. Ctra. Poble Nou Km 5.5, Sant Carles de la Ràpita, 43540 Tarragona, Spain
2 Institute of Environment and Marine Science Research (IMEDMAR-UCV), Universidad Católica de Valencia SVM,C/Explanada del Puerto S/n, Calpe, 03710 Alicante, Spain
3 Instituto de Acuicultura Torre de la Sal, Consejo Superior de Investigaciones Científicas, 12595 Ribera de Cabanes (Castellón), Spain
4 University of Naples Federico II, Department of Biology, Corso Umberto I 40, 80138 Naples, Italy
* Corresponding author: firstname.lastname@example.org
Handling Editor: Pauline Kamermans
Accepted: 11 March 2022
Dwarf oysters in the Ebro Delta are only observed growing on local pen shells, Pinna nobilis L., currently under extinction risk. The species identification of these populations is uncertain, given recent genetic classifications of dwarf oysters of Ostrea stentina species complex. Hence, the first objective of this study was to confirm the identity of dwarf oysters associated to P. nobilis in the Ebro Delta. Then, we aimed to assess the viability of hatchery and nursery production of O. stentina for potential conservation programs using diets based on a mix of live microalgae species or the commercial Shellfish Diet 1800®. Our results showed that COI sequences obtained were specific to O. stentina. Significant differences in the growth and survival of larvae between diet treatments were observed in 3 days. For the live diet, the larval period lasted from 15 to 22 days (299.2 ± 4 µm) whereas it took up to 36 days using the Shellfish Diet 1800® (280 ± 7.2 µm), with an overall mortality of 72.3 vs. 99.3%, respectively for each diet. Besides, no seed survival was observed for the commercial diet after a 10-day-fixation attempt. In contrast, ca. 16% of the individuals on the live diet that were allowed to settle reached the juvenile stage and could be released to the environment. Histological examination of obtained seed did not present any sign of disease and showed gonadal development for both sexes at ca. 6 months of age (16.1–19.1 mm). The growth curve obtained was fitted to a Schnute-Richards growth model which returned ages of up to 10 years for maximum local sizes of 45 mm. We conclude that although the Shellfish Diet 1800® is not an adequate diet for O. stentina, its hatchery production is feasible and might provide support to future conservation actions.
Key words: Ostrea stentina / DNA barcoding / Shellfish Diet 1800® / microalgae diets / Schnute-Richards growth equation / age at first maturity
© P. Prado et al., Published by EDP Sciences 2022
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