Flow chart diagram summarizing the four methods used to estimate energy indices (energy density in kJ.g⁻¹ WW and total energy in kJ). All estimates were compared to energy density measured by bomb calorimetry. Solid lines indicate that a linear (or log-log linear) regression was used to estimate the variables. Dashed lines indicate that an equation was used to estimate the variables. Equation number is indicated in the circles. Methods 2 and 3 are decomposed in three steps corresponding to the estimation of proximate components (step 1), calculation of lipid or protein (step 2) and calculation of energy indices (step 3). L = length, W = weight, Kn = relative condition factor, P = protein mass, F = lipid mass, A = ash mass, H = water mass, HC = water content, ED = energy density, Etot = total energy. Note that m = measured, calc = calculated, est = estimated.