Aquat. Living Resour.
Volume 23, Number 1, January-March 2010
|Page(s)||103 - 107|
|Published online||17 March 2010|
Isolation and use of microsatellite loci in Melicertus kerathurus (Crustacea, Penaeidae)
Dipartimento di Biologia Animale “G. Riverberi”, Università
degli studi di Palermo, Via Archirafi 18, 90123 Palermo, Italy
2 Institut des Sciences de l'Evolution, CNRS UMR 5554, Université de Montpellier II, Station méditerranéenne de l'Environnement littoral, 1 quai de la Daurade, Sète 34200, France
Corresponding author: firstname.lastname@example.org
Accepted: 17 November 2009
Melicertus kerathurus represents an economically important resource for fisheries and aquaculture. Seven microsatellite loci from 373 specimens of M. kerathurus collected in different parts of the Mediterranean and NE Atlantic were identified. Four of these microsatellites showed a moderate level of polymorphism, with 2 to 14 alleles per locus, whereas three had a monomorphic profile. Observed and expected heterozygosity ranged between 0.28 to0.64 and 0.28 to 0.65, respectively. Three microsatellite loci deviated from Hardy-Weinberg equilibrium in some populations, with a deficit of heterozygosity. Mean Fst values showed significant differentiation among sample sites analysed and indicate that these loci are useful for the study of genetic variation in this species. These results may supply functional information on the population genetic structure of Melicertus kerathurus, valuable for future sustainable management strategies.
Key words: Microsatellites / Genetic variation / PCR–RFLP / Penaeid shrimp / Melicertus kerathurus / Mediterranean Sea / Atlantic Ocean
© EDP Sciences, IFREMER, IRD, 2010
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