Free Access
Issue
Aquat. Living Resour.
Volume 27, Number 3-4, July-December 2014
Page(s) 147 - 152
DOI https://doi.org/10.1051/alr/2014015
Published online 15 January 2015
  • Allam B.,Paillard C.,Auffret M., 2000, Alterations in hemolymph and extrapallial fluid parameters in the Manila clam, Ruditapes philippinarum, challenged with the pathogen Vibrio tapetis. J. Invertebr. Pathol. 76, 63–69. [CrossRef] [PubMed]
  • Andersen C.L.,Jensen J.L.,Orntoft T.F., 2004, Normalization of real-time quantitative reverse transcription-PCR data: a model-based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data sets. Cancer Res. 64, 5245–5250. [CrossRef] [PubMed]
  • Araya M.T.,Siah A.,Mateo D.,Markham F.,McKenna P.,Johnson G.,Berthe F.C.J., 2008, Selection and evaluation of housekeeping genes for haemocytes of soft-shell clams (Mya arenaria) challenged with Vibrio splendidus. J. Invertebr. Pathol. 99, 326–331. [CrossRef] [PubMed]
  • Balseiro P.,Moreira R.,Chamorro R.,Figueras A.Novoa B., 2013, Immune responses during the larval stages of Mytilus galloprovincialis: metamorphosis alters immunocompetence, body shape and behavior. Fish Shellfish Immunol. 35, 438–447. [CrossRef] [PubMed]
  • Bustin S.A.,Benes V.,Garson J.A.,Hellemans J.,Huggett J.,Kubista M.,Mueller R.,Nolan T.,Pfaffl M.W.,Shipley G.L.,Vandesompele J.,Wittwer C.T., 2009, The MIQE Guidelines: Minimum information for publication of quantitative real-time PCR Experiments. Clin. Chem. 55, 611–622. [CrossRef] [PubMed]
  • Costa M.M.,Novoa B.,Figueras A., 2008, Influence of β-glucans on the immune responses of carpet shell clam (Ruditapes decussatus) and Mediterranean mussel (Mytilus galloprovincialis). Fish Shellfish Immunol. 24, 498–505. [CrossRef] [PubMed]
  • Costa M.M.,Prado-Alvarez M.,Gestal C.,Li H.,Roch P.,Novoa B.,Figueras A., 2009, Functional and molecular immune response of Mediterranean mussel (Mytilus galloprovincialis) haemocytes against pathogen-associated molecular patterns and bacteria. Fish Shellfish Immunol. 26, 515–523. [CrossRef] [PubMed]
  • Cubero-Leon E.,Ciocan C.M.,Miner C.,Rotchell J.M., 2012, Reference gene selection for qPCR in mussel, Mytilus edulis, during gametogenesis and exogenous estrogen exposure. Environ. Sci. Pollut. Res. Int. 19, 2728–2733. [CrossRef]
  • Dheilly N.M.,Lelong C.,Huvet A.,Favrel P., 2011, Development of a Pacific oyster (Crassostrea gigas) 31,918-feature microarray: identification of reference genes and tissue-enriched expression patterns. BMC Genomics 12, 468. [CrossRef] [PubMed]
  • Du Y.,Zhang L.,Xu F.,Huang B.,Zhang G.,Li L., 2013, Validation of housekeeping genes as internal controls for studying gene expression during Pacific oyster (Crassostrea gigas) development by quantitative real-time PCR. Fish Shellfish Immunol. 34, 939–945. [CrossRef] [PubMed]
  • García-Vallejo J.J., Van Het Hof B.,Robben J., Van Wijk J.A., Van Die I.,Joziasse D.H., Van Dijk W., 2004, Approach for defining endogenous reference genes in gene expression experiments. Anal. Biochem. 329, 293–299. [CrossRef] [PubMed]
  • Gestal C.,Roch P.,Renault T.,Pallavicini A.,Paillard C.,Novoa B.,Oubella R.,Venier P.,Figueras A., 2008, Study of diseases and the immune system of bivalves using molecular biology and genomics. Rev. Fish. Sci. 16, 131–114. [CrossRef]
  • Gómez-León J.,Villamil L.,Lemos M.L.,Novoa B.,Figueras A., 2005, Isolation of V. alginolyticus and Vibrio splendidus from aquacultured carpet shell clam (Ruditapes decussatus) larvae associated with mass mortalities. Appl. Environ. Microbiol. 71, 98–104. [CrossRef] [PubMed]
  • Gueguen Y.,Cadoret J.P.,Flament D.,Barreau-Roumiguiere C.,Girardot A.L.,Garnier J.,Hoareau A.,Bachere E.,Escoubas J.M., 2003, Immune gene discovery by expressed sequence tags generated from hemocytes of the bacteria-challenged oyster, Crassostrea gigas. Gene 303, 139–145. [CrossRef] [PubMed]
  • Huggett J.,Dheda K.,Bustin S.,Zumla A., 2005, Real-time RT-PCR normalisation; strategies and considerations. Genes Immunity 6, 279–284. [CrossRef] [PubMed]
  • Li H.,Venier P.,Prado-Alvarez M.,Gestal C.,Toubiana M.,Quartesan R.,Borghesan F.,Novoa B.,Figueras A.,Roch P., 2010, Expression of Mytilus immune genes in response to experimental challenges varied according to the site of collection. Fish Shellfish Immunol. 28, 640–648. [CrossRef] [PubMed]
  • Martínez-Fernández M.,Bernatchez L., Rolán-Alvarez E.,Quesada H., 2010, Insights into the role of differential gene expression on the ecological adaptation of the snail Littorina saxatilis. BMC Evol. Biol. 10, 356. [CrossRef] [PubMed]
  • Martins E.,Figueras A.,Novoa B.,Santos R.S.,Moreira R.,Bettencourt R., 2014, Comparative study of immune responses in the deep-sea hydrothermal vent mussel Bathymodiolus azoricus and the shallow-water mussel Mytilus galloprovincialis challenged with Vibrio bacteria. Fish Shellfish Immunol. 40, 485–499. [CrossRef] [PubMed]
  • Mauriz O.,Maneiro V.,Pérez-Parallé M. L.,Sánchez J. L.,Pazos A. J., 2012, Selection of reference genes for quantitative RT-PCR studies on the gonad of the bivalve mollusc Pecten maximus L. Aquaculture 370-371, 158–165. [CrossRef]
  • Mitter K.,Kotoulas G.,Magoulas A.,Mulero V.,Sepulcre P.,Figueras A.,Novoa B.,Sarrapoulou E., 2009, Evaluation of candidate reference genes for qPCR during ontogenesis and of immune-relevant tissues of European seabass (Dicentrarchus labrax). Comp. Biochem. Physiol. B 153, 340–347. [CrossRef]
  • Moreira R.,Balseiro P.,Romero A.,Dios S.,Posada D.,Novoa B.,Figueras A., 2012a, Gene expression analysis of clams Ruditapes philippinarum and Ruditapes decussatus following bacterial infection yields molecular insights into pathogen resistance and immunity. Dev. Comp. Immunol. 36, 140–149. [CrossRef] [PubMed]
  • Moreira R.,Balseiro P.,Planas J.V.,Fuste B., Beltran S.,Novoa B.,Figueras A., 2012b, Transcriptomics of in vitro immune-stimulated hemocytes from the Manila clam Ruditapes philippinarum using high-throughput sequencing. Plos One 7, e35009. [CrossRef] [PubMed]
  • Moreira R.,Milan M.,Balseiro P.,Romero A., Babbucci M.,Figueras A.,Bargelloni L.,Novoa B, 2014, Gene expression profile analysis of Manila clam (Ruditapes philippinarum) hemocytes after a Vibrio alginolyticus challenge using an immune-enriched oligo-microarray. BMC Genomics 15, 267. [CrossRef] [PubMed]
  • Pfaffl M.W., 2001, A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res. 29, e45. [CrossRef]
  • Pfaffl M.W.,Tichopad A.,Prgomet C.,Neuvians T.P., 2004, Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: BestKeeper-Excel-based tool using pair-wise correlations. Biotechnol. Lett. 26, 509–515. [CrossRef] [PubMed]
  • Romero A.,Dios S.,Poisa-Beiro L.,Costa M.M.,Posada D.,Figueras A.,Novoa B., 2011, Individual sequence variability and functional activities of fibrinogen-related proteins (FREPs) in the Mediterranean mussel (Mytilus galloprovincialis) suggest ancient and complex immune recognition models in invertebrates. Dev. Comp. Immunol. 35, 334–344. [CrossRef] [PubMed]
  • Rozen S.,Skaletsky H.J., 2000, Primer3 on the WWW for general users and for biologist programmers. Meth. Mol. Biol. 132, 365–386.
  • Siah A.,Dohoo C.,McKenna P.,Delaporte M.,Berthe F.C.J., 2008, Selecting a set of housekeeping genes for quantitative real-time PCR in normal and tetraploid haemocytes of soft-shell clams, Mya arenaria. Fish Shellfish Immunol. 25, 202–207. [CrossRef] [PubMed]
  • Silver N.,Best S.,Jiang J.,Thein S.L., 2006, Selection of housekeeping genes for gene expression studies in human reticulocytes using real-time PCR. BMC Mol. Biol. 7, 33. [CrossRef] [PubMed]
  • Vandesompele J., De Preter K., Pattyn F., Poppe B., Van Roy N., De Paepe A., Speleman F., 2002, Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol. 3, Research0034.
  • Waki T.,Shimokawa J.,Watanabe S.,Yoshinaga T.,Ogawa K., 2012, Experimental challenges of wild Manila clams with Perkinsus species isolated from naturally infected wild Manila clams. J. Invertebr. Pathol. 111, 50–55. [CrossRef] [PubMed]
  • Winnebeck E.C.,Millar C.D.,Warman G.R., 2010, Why does insect RNA look degraded? J. Insect Sci. 10, 159. [CrossRef] [PubMed]
  • Wu H.,Ji C.,Wei L.,Zhao J.,Lu H., 2013, Proteomic and metabolomic responses in hepatopancreas of Mytilus galloprovincialis challenged by Micrococcus luteus and Vibrio anguillarum. J. Proteomics 94, 54–67. [CrossRef] [PubMed]

Current usage metrics show cumulative count of Article Views (full-text article views including HTML views, PDF and ePub downloads, according to the available data) and Abstracts Views on Vision4Press platform.

Data correspond to usage on the plateform after 2015. The current usage metrics is available 48-96 hours after online publication and is updated daily on week days.

Initial download of the metrics may take a while.