Total replacement of soybean meal by rice distilled dried grain soluble (DDGS) with lysine supplementation: effect on growth, nucleic acid content, digestive enzyme activity, and health status in Carp (Cirrhinus mrigala)

Open Access

Table 7

Hematological parameters of C. mrigala juveniles fed with different experimental diet at the end of the experiment.

Treatments	Hemoglobin content (g/dl)	Total erythrocyte count (10⁶ cells/mm³)	Total leucocyte count (10⁴ cells/mm³)	Total protein (g/dl)	Albumin (A) (g/dl)	Globulin (G) (g/dl)	A/G ratio
C	5.13^b ± 0.14	1.35^d ± 0.04	15.68^abc ± 0.22	5.29^ef ± 0.13	1.30 ± 0.15	3.98^ef ± 0.04	0.32^abcde ± 0.04
D₆	5.66^b ± 0.26	1.92^abc ± 0.03	17.97^a ± 0.10	6.28^cd ± 0.17	1.25 ± 0.05	5.03^bc ± 0.18	0.25^cde ± 0.01
D₁₂	5.70^b ± 0.17	1.89^bc ± 0.01	17.56^ab ± 0.73	6.26^cd ± 0.07	1.49 ± 0.24	4.76^cd ± 0.31	0.32^abcde ± 0.06
D₁₈	4.40^d ± 0.20	1.41^d ± 0.07	16.59^ab ± 1.61	5.19^ef ± 0.14	1.44 ± 0.19	3.76^efg ± 0.29	0.39^ab ± 0.07
D₂₄	5.10^bc ± 0.11	1.69^c ± 0.07	15.22^bc ± 0.06	4.84^fg ± 0.33	1.45 ± 0.05	3.39^fg ± 0.35	0.44^a ± 0.05
D₃₀	4.33^d ± 0.20	1.21^d ± 0.05	13.97^c ± 0.09	4.44^g ± 0.20	1.33 ± 0.06	3.11^g ± 0.16	0.43^ab ± 0.01
CL	5.73^b ± 0.18	1.95^ab ± 0.05	15.74^abc ± 0.15	7.04^b ± 0.28	135 ± 0.07	5.69^b ± 0.32	0.24^de ± 0.02
D₆L	6.66^a ± 0.38	2.02^a ± 0.19	17.68^ab ± 0.62	7.72^a ± 0.26	1.24 ± 0.14	6.48^a ± 0.16	0.19^e ± 0.02
D₁₂L	6.70^a ± 0.32	2.02^a ± 0.01	17.43^ab ± 0.37	6.67^bc ± 0.24	1.51 ± 0.09	5.16^bc ± 0.17	0.29^bcde ± 0.01
D₁₈L	5.06^bc ± 0.08	1.76^bc ± 0.03	16.52^ab ± 1.64	5.73^de ± 0.27	1.40 ± 0.16	4.33^de ± 0.18	0.32^abcde ± 0.03
D₂₄L	5.33^b ± 0.14	1.72^bc ± 0.03	15.42^bc ± 0.12	5.06^efg ± 0.11	1.32 ± 0.11	3.74^efg ± 0.03	0.35^abcd ± 0.02
D₃₀L	5.03^bc ± 0.09	1.72^bc ± 0.06	14.04^c ± 0.15	5.09^efg ± 0.17	1.40 ± 0.11	3.69^efg ± 0.18	0.38^abc ± 0.04
Variation source	Two way ANOVA
Level of rice DDGS	P < 0.05	P < 0.05	P < 0.05	P < 0.05	NS	P < 0.05	P < 0.05
With or without lysine	P < 0.05	P < 0.05	NS	P < 0.05	NS	P < 0.05	P < 0.05
Interaction	P < 0.05	P < 0.05	P < 0.05	P < 0.05	NS	P < 0.05	P < 0.05

Data are expressed as mean ± S.E. where n = 3.

Mean values in the same column with different superscripts vary significantly (P < 0.05).

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