Mixed leaf meal supplemented with exogenous enzyme and limiting amino acids can completely replace DORB (de-oiled rice bran) in the diet of Labeo rohita

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Table 2

Chemical composition and anti-nutritional factors of different leaf meal and mixed leaf meal (LM).

Chemical composition	Vigna mungo	Ipomoea aquatica	Hygrophila spinosa	Mixed leaf meal	DORB
Proximate composition (g kg^–1 dry matter basis)
Dry matter	917.1 ± 0.08	937.9 ± 0.16	923.8 ± 0.08	942.6 ± 0.17	938.2 ± 0.10
Crude protein	238.5 ± 0.17	231.2 ± 0.12	221.9 ± 0.15	235.4 ± 0.17	160.0 ± 0.20
Ether extract	24.3 ± 0.03	21.4 ± 0.09	20.9 ± 0.12	23.0 ± 0.07	12.0 ± 0.90
Crude fibre	160.2 ± 0.05	162.1 ± 0.11	166.0 ± 0.08	162.7 ± 0.29	137.3 ± 0.26
Nitrogen free extract	456.2 ± 0.06	454.1 ± 0.06	449.2 ± 0.04	441.5 ± 0.22	500.2 ± 0.28
Total ash	120.8 ± 0.04	131.2 ± 0.13	142.0 ± ± 0.04	137.4 ± 0.09	122.0 ± 0.27
Anti-nutritional factors and IVCPD¹
Total tannins (g 100g^–1)	0.36	0.94	0.49	0.52	0.34
Alkaloid (g 100g^–1)	0.31	0.73	1.82	0.68	0.23
Total oxalate (g 100g^–1)	0.005	0.83	0.41	0.39	0.008
Phytic acid (mg 100g^–1)	0.06	4.63	5.81	3.43	2.69
IVCPD¹ (%)	-	-	-	28.42	29.64

Data are expressed as mean ± SEM (standard error of mean).

¹IVCPD, in vitro crude protein digestibility.

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