Table 1
Formulation, proximate composition and anti-nutritional factors of experimental diets
| Ingredients (g kg–1) | 1Experimental diets | |||||
|---|---|---|---|---|---|---|
| C | LM20 | LM40 | CE* | LM20E* | LM40E* | |
| De-fatted soybean meal | 195.00 | 157.50 | 120.00 | 200.00 | 161.00 | 125.00 |
| Groundnut oil cake | 300.00 | 300.00 | 300.00 | 300.00 | 300.00 | 300.00 |
| Wheat flour | 23.80 | 61.30 | 98.80 | 15.00 | 55.82 | 91.30 |
| De-oiled rice bran | 400.00 | 200.00 | 0.00 | 400.00 | 200.00 | 0.00 |
| Mixed leaf meal | 0.00 | 200.00 | 400.00 | 0.00 | 200.00 | 400.00 |
| Sunflower oil | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Fish oil | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Vitamin-mineral mix2 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Betaine | 5.00 | 5.00 | 5.00 | 5.00 | 5.00 | 5.00 |
| Choline chloride | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 | 1.00 |
| Carboxymethyl cellulose | 15.00 | 15.00 | 15.00 | 15.00 | 15.00 | 15.00 |
| Butylated hydroxytoluene | 0.20 | 0.20 | 0.20 | 0.20 | 0.20 | 0.20 |
| Exogenous enzyme3 | 0.00 | 0.00 | 0.00 | 1.00 | 1.00 | 1.00 |
| DL-Methionine* | 0.00 | 0.00 | 0.00 | 1.40 | 0.00 | 0.00 |
| L-Lysine* | 0.00 | 0.00 | 0.00 | 1.40 | 0.98 | 1.50 |
| Total | 1,000 | 1,000 | 1,000 | 1,000 | 1,000 | 1,000 |
| Chromium oxide (Cr2O3) | 5.00 | 5.00 | 5.00 | 5.00 | 5.00 | 5.00 |
| Proximate composition (g kg–1 dry matter basis) | ||||||
| Dry matter | 911.8 | 911.5 | 913.0 | 909.0 | 908.0 | 905.7 |
| Crude protein | 301.0 | 301.0 | 301.2 | 302.1 | 301.7 | 301.30 |
| Ether extract | 58.00 | 59.00 | 59.60 | 58.30 | 61.00 | 59.20 |
| Crude fiber | 51.00 | 58.00 | 64.00 | 33.0 | 41.2 | 53.10 |
| Nitrogen free extract | 515.3 | 504.6 | 497.3 | 530.2 | 517.9 | 511.5 |
| Total ash | 74.70 | 77.40 | 77.90 | 76.40 | 78.20 | 75.00 |
| GE4 (MJ kg–1) | 17.21 | 17.42 | 17.62 | 17.00 | 17.24 | 17.44 |
| DE5 (MJ kg–1) | 15.83 | 15.68 | 15.59 | 16.10 | 15.99 | 15.81 |
| P: E6 (g protein MJ DE–1) | 18.95 | 19.17 | 19.31 | 18.75 | 18.85 | 19.03 |
| Anti-nutritional factors | ||||||
| Total tannins (g 100g–1) | 0.07 | 0.22 | 0.32 | 0.06 | 0.23 | 0.31 |
| Alkaloid (g 100g–1) | 0.11 | 0.24 | 0.32 | 0.11 | 0.25 | 0.33 |
| Total oxalate (g 100g–1) | 0.06 | 0.13 | 0.19 | 0.07 | 0.13 | 0.20 |
| Phytic acid (mg 100g–1) | 0.05 | 1.83 | 2.81 | 0.05 | 1.82 | 2.80 |
Data (proximate composition and anti-nutritional factors) are expressed as mean values of triplicate. Please shift this 1 to next line like 1 C, Control with 0 g kg–1 mixed leave meal (LM) and C, Control with 0 g kg–1 mixed leave meal (LM) and 400 g kg–1 de-oiled rice bran (DORB); LM20, 200 g kg–1 LM in replacement of 50% DORB; LM40, 400 g kg–1 LM in replacement of 100% DORB; CE, Control diet supplemented with 1 g kg–1 xylanase and cellulase enzyme mixture; LM20E, LM20 diet supplemented with 1 g kg–1 xylanase and cellulase enzyme mixture; LM40E, LM40 diet supplemented with 1 g kg–1 xylanase and cellulase enzyme mixture.
CE, LM20E and LM40E were supplemented with 1.4 g kg–1 DL-methionine and 1.4 g kg–1 L-lysine, 0.98 g kg–1 L-lysine and 1.5 g kg–1 L-lysine, respectively to fulfill the essential amino acid requirements of L. rohita (FAO, 2017).
Composition of vitamin-mineral mix (AGRIMIN FORTE) (quantity kg–1): vitamin A, 7,00,000 IU; vitamin D3, 70,000 IU; vitamin E, 250 mg; nicotinamide, 1000 mg; cobalt, 150 mg; copper, 1200 mg; iodine, 325 mg; iron, 1500 mg; magnesium, 6000 mg; manganese, 1500 mg; potassium, 100 mg; sodium, 5.9 mg; selenium, 7200 mg; zinc, 9600 mg; calcium, 255 g; phosphorus, 127.5 g.
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