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Table 1
Primer pairs targeting the mitochondrial cytochrome b of Ostrea edulis, Crassostrea gigas, and Mytilus edulis. The reverse primers are labelled at 5′ with a specific dye, respectively the forward primers are labelled at 5′ with biotin for later implementation to nucleic acid lateral flow immunoassay.
Primer: | Sequence: | 5′−end label: |
---|---|---|
O. edulis forward | TAA AGT ATC CCT GTC CGC CC | Biotin |
O. edulis reverse | AAC CCT CCG AAT AGC TCA CC | FITC |
C. gigas forward | TCA TAA AGC AGG TGG GGT GT | Biotin |
C. gigas reverse | TCC TGA GCA CCA ATA ACC GT | DY-632 |
M. edulis forward | GCC TAT TCA CGT TCA GCC TG | Biotin |
M. edulis reverse | GGA CGA GCA CCA ATT CAT GT | DY-495 |
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