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Table 2
Characteristics of the nine amplified microsatellite loci used in this study for population analysis of the tarek, Alburnus tarichi.
| Locus | Primer sequence (5′–3′) | Core motif | Fluorescent dye | Size (bp) | NA | Ta (°C) |
|---|---|---|---|---|---|---|
| Lsou08 1 | F: GCGGTGAACAGGCTTAACTC R: TAGGAACGAAGAGCCTGTGG |
(GT)17 | PET-5' | 187–195 | 5 | 56–58* |
| LceD63 2 | F: ATGGCAGGTTAAGTGGATTG R: TGGTGGATGGAACACAATC |
(GT)43 | NED-5' | 122–160 | 12 | 56–58* |
| Lsou34 1 | F: CCAGACAGGGTGATGATTCC R: GTAGCGACGTTCAGGTCTCG |
(GT)15 | NED-5' | 228–278 | 10 | 56–58* |
| LceC172 2 | F: CAACTAAAAGGGGATTAATG R: GTAACATGAACAAACAATGAAC |
(TG)19(TA)10 | VIC-5' | 230–244 | 6 | 50 |
| LceDT 2 | F: CTTGAATGCTATAATGCTCTTGG R: CACCCCGTCATTGTGTTC |
(GT)22(AT)16 | 6-FAM-5' | 128–166 | 9 | 58 |
| Lco3 3 | F: GCAGGAGCGAAACCATAAAT R: AAACAGGCAGGACACAAAGG |
(TG)9 | 6-FAM-5' | 248–256 | 9 | 58 |
| Lsou05 1 | F: CTGAAGAAGACCCTGGTTCG R: CCCACATCTGCTGACTCTGAC |
(CA)17 | VIC-5' | 178–200 | 6 | 58 |
| BL1-2b 4 | F: TTTGCACTAGTAACGAGCATCA R: CAGCACAGTTTCTCCATCCA |
(TG)12 | NED −5' | 142-176 | 13 | 58 |
| BL1-61 4 | F: GACTCGCAGAGCTCCTTCAC R: GGCGCTTGATTCAGTCTTTC |
(CA)8 | PET −5' | 192-228 | 12 | 58 |
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