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Table 1
List of BOLD and GenBank screening results. Highlighted are the erroneous sequences from GenBank or BOLD offered for maximum identity scores.
| Sample name | ID based on morphology | ID based on COI barcode | BOLD similarity | BOLD species | GenBank ident. | GenBank E-value | GenBank species |
|---|---|---|---|---|---|---|---|
| Str_4.5, Str_3.3, Str_3.2, Str_3.1, I11, Str_3.18, Str_4.18, Str_5.16, Str_5.24, Str_3.4, Str_4.4, Str_4.2, Str_4.1, Str_3.5, Str_4.7, Str_5.28, Str_5.1, Str_5.12 | Salmo trutta fario (except I11 which was initially identified as Oncorhynchus mykiss) | Salmo trutta fario | 100% | Salmo farioides | 100% | 0.0 | Salmo farioides |
| Lo5 | Hucho hucho | Salmo trutta fario | 100% | Salmo trutta, Salmo obtusirostris, Salmo fibreni, Salmo letnica, Salmo farioides, Salmo zrmanjaensis, Salmo visovacensis | 100% | 0.0 | Salmo trutta fario, Salmo labrax, Salmo trutta caspius, Salmo trutta trutta, Salmo zrmanjaensis, Salmo fibreni, Salmo letnica, Salmo visovacensis, Salmo obtusirostris, Salmo trutta |
| I9 | Salmo trutta | Salmo trutta fario | 100% | Salmo labrax | 100% | 0.0 | Salmo labrax |
| Str_3a, I6 | Salmo trutta | Salmo trutta fario | 100% | Salmo trutta | 100% | 0.0 | Salmo trutta, Salmo trutta fario, Salmo Pellegrini, Salmo cettii, Salmo trutta trutta |
| L1_FM | Salmo salar | Salmo salar | 100% | Salmo salar | 100% | 0.0 | Salmo salar |
| Hh_Lo | Hucho hucho | Hucho hucho | 100% | Hucho hucho | 100% | 0.0 | Hucho hucho, Leucaspius delineatus |
| Sfo_4a, Sfo_II3, Sfo_I5, Sfo_I7, Sfo_I2, B3_FM, Sfo_II6 | Salvelinus fontinalis (except the B3_FM sample that was labelled as Oncorhynchus mykiss) | Salvelinus fontinalis | 100% | Salvelinus fontinalis | 100% | 0.0 | Salvelinus fontinalis |
| Li10 | Thymallus thymallus | Salvelinus fontinalis | 99.76% | Salvelinus fontinalis | 99% | 0.0 | Salvelinus fontinalis |
| Omy_9a, Omy_18a, Omy_17a, Omy_16a, Omy_13a, Omy_10a, Omy_14a, Omy_4a, Omy_3a, Omy_1a, Omy_20a, Omy_12a, Omy_19a, Omy_8a, Omy_7a, Omy_6a, Omy_5a, Omy_2a, Omy_II2, Omy_02-Damb, Omy_05-Cand, Omy_09-1m, Omy_09-3m, Omy_09-5m, L2_FM, L3_FM, M1_FM, I4, I3 | Oncorhynchus mykiss (except the I4 and I3 samples which were initially labelled as Salmo trutta) | Oncorhynchus mykiss | 100% | Oncorhynchus mykiss | 100% | 0.0 | Oncorhynchus mykiss |
| M2_FM | Unlabelled | Oncorhynchus mykiss | 99.76% | Oncorhynchus mykiss | 99% | 0.0 | Oncorhynchus mykiss |
| B2_FM | Oncorhynchus mykiss | Oncorhynchus mykiss | 98.55% | Oncorhynchus mykiss | 99% | 0.0 | Oncorhynchus mykiss |
| Tthy_Li | Thymallus thymallus | Thymallus thymallus | 100% | Thymallus aeliani | 100% | 0.0 | Thymallus aeliani |
| Ast_184, Ast_185, Ast_186, Ast_187, Ast_188 | Acipenser stellatus | Acipenser stellatus | 100% | Acipenser stellatus | 100% | 0.0 | Acipenser nudiventris, Acipenser stellatus |
| Ast_1886, Ast_4140, Ast_8038, Ast_7188, Ast_9296, Ast_1818 | Acipenser stellatus | Acipenser stellatus | 100% | Acipenser stellatus | 100% | 0.0 | Acipenser stellatus |
| Agu_8_17_18 | Acipenser gueldenstaedtii | Acipenser gueldenstaedtii | 100% | Huso huso, Acipenser gueldenstaedtii | 100% | 0.0 | Acipenser gueldenstaedtii, Acipenser naccarii, Acipenser gueldenstaedtii × Huso dauricus, Acipenser sinensis, Acipenser persicus |
| Agu_61, Agu_62, Agu_65, Agu_70, Agu_72, Agu_285, Agu_284, Agu_271, Agu_273 | Acipenser gueldenstaedtii | Acipenser gueldenstaedtii | 100% | Huso huso, Acipenser gueldenstaedtii | 100% | 0.0 | Acipenser gueldenstaedtii, Acipenser naccarii, Acipenser gueldenstaedtii × Huso dauricus, Acipenser gueldenstaedtii × Acipenser baerii, Acipenser sinensis, Acipenser persicus |
| Abae_81, Abae_84, Abae_86, Abae_93, Abae_95, Abae_96 | Acipenser baerii | Acipenser baerii | 100% | Acipenser baerii | 100% | 0.0 | Acipenser gueldenstaedtii, Acipenser baerii, Acipenser baerii × Huso dauricus, Acipenser baerii × Acipenser schrenckii, Acipenser persicus |
| Begu_99, Begu_100, Begu_112, Begu_113 | Sturgeon hybrid, ♀ A. baerii × ♂ A. gueldenstaedtii | Placed in the clade with A. baerii, A. persicus and A. gueldenstaedtii | 100% | Acipenser baerii | 100% | 0.0 | Acipenser gueldenstaedtii, Acipenser baerii, Acipenser baerii × Huso dauricus, Acipenser baerii × Acipenser schrenckii, Acipenser persicus |
| Hh_742, Hh_743, Hh_280, Hh_281, Hh_282, Hh_1, Hh_12, Hh_18, Hh_20_F, Br_1_FM, Br_2_FM, Bester_5, Bester_9, Bb_RS, Bb_RL, Bb_4, Bb_9 | Huso huso (except the Br_1_FM and Br_2_FM samples which were labelled as “sturgeon”; Bester_5 and Bester_9 samples were labelled as Sturgeon hybrid, ♀ H. huso × ♂ A. ruthenus; Bb_RS, Bb_RL, Bb_4, and Bb_9 were labelled as sturgeon hybrid, ♀ (H. huso × ♂ A. ruthenus) × ♂ H. huso) | Huso huso | 100% | Huso huso | 100% | 0.0 | Acipenser ruthenus × Huso dauricus, Huso huso |
| Ar_144_FM, Ar_148_FM, Ar_8112_FM, Aru_250, Aru_251, Aru_252 | Acipenser ruthenus (except Ar_144_FM, Ar_148_FM, and Ar_8112_FM which were labelled as “sturgeon”) | Acipenser ruthenus | 100% | Huso dauricus, Acipenser ruthenus | 100% | 0.0 | Acipenser ruthenus |
| Sterbe_1, Sterbe_2, Sterbe_3, Sterbe_4 | Sturgeon hybrid, ♀ A. ruthenus × ♂ H. huso | Acipenser ruthenus | 100% | Huso dauricus, Acipenser ruthenus, Acipenser ruthenus × baerii | 100% | 0.0 | Acipenser ruthenus |
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