Aquat. Living Resour.
Volume 26, Number 3, July-September 2013
Thematic Section: Physiology in Marine Molluscs
|Page(s)||207 - 213|
|Published online||06 May 2013|
Multiplex PCR sets of novel microsatellite loci for the great scallop Pecten maximus and their application in parentage assignment
1 Institut Universitaire Européen de la
Mer, Laboratoire des sciences de l’environnement marin, LEMAR, UMR 6539,
rue Dumont d’Urville,
2 Ifremer, Laboratoire de génétique et pathologie des mollusques marins, 17390 La Tremblade, France
3 Department of Biological and Environmental Sciences – Tjärnö, University of Gothenburg, 45296 Strömstad, Sweden
4 Université de Montpellier II, UMR CNRS 5554, UMR 226, Institut des Sciences de l’Evolution de Montpellier, Place E. Bataillon, cc63, 34095 Montpellier Cedex 5, France
5 Ifremer, Laboratoire des sciences de l’environnement marin, BP 70, 29280 Plouzané, France
a Corresponding author:
Accepted: 20 March 2013
We report the isolation, development and multiplex optimisation of 12 new microsatellite loci for the great scallop, Pecten maximus. Diversity was moderate to high, with number of alleles ranging from 4 to 20 and observed heterozygosity between 0.28 and 0.88. Progeny produced in a commercial hatchery was used to test locus power for parentage assignment. The percentage of offspring that was unambiguously assigned to a unique pair of parents was 97% (software package CERVUS-COLONY). Parentage assignment revealed that 22% of the studied progeny resulted from unplanned crosses. Effective population size of the study progeny was also estimated. Our study illustrates the power of microsatellites for the genetic monitoring of hatchery-produced great scallops.
Key words: Microsatellites / Polymerase chain reaction / Parentage / Assignment / Bivalve mollusc
© EDP Sciences, IFREMER, IRD 2013
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