Aquat. Living Resour.
Volume 25, Number 2, April-June 2012
|Page(s)||185 - 194|
|Published online||16 July 2012|
Cultured heart cells from oyster : an experimental approach for evaluation of the toxicity of the marine pollutant tributyltin
EA 1274 M2S, Facteurs nerveux et Structuration tissulaire, UFR
Médecine et Sciences de la Santé, 22 avenue Camille Desmoulins, 29238
2 IFREMER, Centre de Brest, BP 70, 29280 Plouzané Cedex, France
3 Irish Centre for Environmental Toxicology, Galway-Mayo Institute of Technology, Dublin Rd, Galway, Ireland
a Corresponding author :
Accepted: 1 June 2012
European Community regulations on chemicals promote alternative methods to test substances presenting potential risks for the environment. In the present work, cultured atrial cells isolated from oyster (Crassostrea gigas) were used as an experimental model to investigate the toxicity of tributyltin (TBT) after short-time exposure at concentrations representative of those that can be measured in seawater, marine sediments and/or bivalves bioaccumulating this pollutant. In vitro and in vivo assays produce values of the same order of magnitude for both animal/cell survival and heart/cardiomyocyte beating rate. The survival rate of whole animals decreased from 10-6 M TBT after 3 days. For cultured cells, the viability, evaluated using 3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide (MTT) assay, significantly decreased after two days of treatment with 10-6 M TBT, and after six days with 10-10 M TBT. The percentage of apoptotic cells, quantified by flow cytometry and YO-PRO®-1 iodide, a nucleic acid stain that only permeates cells that are beginning to undergo apoptosis, increased significantly in these cases. Moreover, intracellular concentration of Ca++ had increased after 10 min of exposition to 10-6 M, and could be associated with apoptotic processes. As patch clamp experiments showed that Ca++ conductance was decreased, intracellular calcium increase could mainly be due to a release from internal stores. The decreases in beating rhythm could be explained by the decrease in adenosine triphosphate (ATP) production revealed by 31 P nuclear magnetic resonance (NMR) spectroscopy and confirmed by the increase of the KATP channel conductance. The related hyperpolarization and the disturbances of the energetic metabolism were clearly related to the loss of the atrial cell contractility and viability.
Key words: Oyster / Heart cells / Flow cytometry / Tributyltin / Toxicity / Antifouling
© EDP Sciences, IFREMER, IRD 2012
Current usage metrics show cumulative count of Article Views (full-text article views including HTML views, PDF and ePub downloads, according to the available data) and Abstracts Views on Vision4Press platform.
Data correspond to usage on the plateform after 2015. The current usage metrics is available 48-96 hours after online publication and is updated daily on week days.
Initial download of the metrics may take a while.