Aquat. Living Resour.
Volume 16, Number 5, October 2003Genetics and Reproduction of Common Carp
|Page(s)||461 - 465|
|Published online||15 October 2003|
Characterization of protease inhibitors of seminal plasma of cyprinids
Department of Semen Biology, Polish Academy of Sciences, 10-718, Olsztyn, Poland
2 Department of Molecular Andrology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, 10-718, Olsztyn, Poland
3 Experimental Fish Farm Zator, 32-640, Zator, Poland
4 Chair of Lake and River Fisheries, University of Warmia and Mazury, 10-718, Olsztyn-Kortowo, Poland
Corresponding author: email@example.com
Accepted: 12 May 2003
Anti-proteinase activity was demonstrated in the seminal plasma of cyprinid fish species (bream, chub, ide, dace, asp, goldfish, roach, common carp) using electrophoretic techniques combined with a detection method based on inhibition of bovine trypsin. We found species-specific protease inhibitors in the seminal plasma of cyprinids. At least three bands of protease inhibitors with different migration rates could be identified by native PAGE. Higher variability was characterized for bands with slower migration rates. Visualization of inhibitors after SDS-PAGE under non-reducing conditions allowed estimation of their molecular weights. Apparent molecular weights were within the range of 51–59 and 47–54 kDa for the bands with slower and moderate migration rates, respectively. The molecular weight of fast migration bands for roach and common carp were estimated to 23 and 30 kDa, respectively. Inhibitors of common carp seminal plasma differed in their affinity toward serine proteases. Three inhibitors in common carp seminal plasma could be visualized using cod and bovine trypsin, but only two inhibitors (of high molecular weight) were recognized with chymotrypsin. There were differences in anti-proteinase activity and seminal plasma protein concentration in relation to the origin of common carp seminal plasma (breeding lines) and time of milt collection (spawning vs. post-spawning season).
Key words: Seminal plasma / Anti-proteinase activity / Cyprinids
© Elsevier, IRD, Inra, Ifremer, Cemagref, 2003
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