| Issue |
Aquat. Living Resour.
Volume 11, Number 1, February 1998
|
|
|---|---|---|
| Page(s) | 45 - 48 | |
| DOI | https://doi.org/10.1016/S0990-7440(99)80030-8 | |
| Published online | 15 February 1998 | |
Long-term effects of the cryopreservation of turbot (Psetta maxima) spermatozoa
1
IFREMER, Laboratoire de Physiologie des Poissons, Centre de Brest, BP70, 29280 Plouzané, France
2
Muséum National d'Histoire Naturelle, Laboratoire d'Ichtyologie, 43 rue Cuvier, 75231 Paris, France
Received:
19
November
1997
Accepted:
15
January
1998
The survival of turbot eggs and the rearing capacities of larvae stemmed from artificial fertilization practices using frozen-thawed spermatozoa were evaluated. Furthermore, the viability of sperm samples stored during a 9 month period in liquid nitrogen was assessed. No significant difference in the fertilization rate, hatching rate, survival and wet weight of 10-day old larvae were observed using fresh or frozen-thawed spermatozoa. The motility recorded at 10 s and 60 s post-activation and the fertilization capacity of frozen-thawed spermatozoa were not significantly decreased during a 9 month storage period in liquid nitrogen. These results confirm the high quality of the turbot spermatozoa stemmed from the cryopreservation process, allowing their use for routine aquaculture practices.
Key words: turbot / Psetta maxima / sperm / cryopreservation / hatching
© Elsevier, IRD, Inra, Ifremer, Cemagref, CNRS, 1998
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