Aquat. Living Resour.
Volume 10, Number 4, July-August 1997
|Page(s)||251 - 255|
|Published online||15 July 1997|
Short-term storage and cryopreservation of turbot (Scophthalmus maximus) sperm
I.E.O., Centro Oceanográfico de Santander, Apdo n 240, 39080 Santander, Spain
2 I.E.O., Centro Oceanográfico de Vigo, Apdo n 1552, 36280 Vigo, Spain
3 IFREMER, Laboratoire de Physiologie des Poissons, Centre de Brest, BP 70, 29280 Plouzané, France
4 INRA, Unité de Physiologie des Poissons, IFR 43, Campus de Beaulieu, 35042 Rennes Cedex, France
5 Author to whom correspondence should be addressed; E-mail: firstname.lastname@example.org
Accepted: 23 April 1997
Short-term storage over several days as well as cryopreservation of turbot (Scophthalmus maximus) sperm were studied. Two extenders, Ringer 200 and artificial seminal liquid (ASL2), are suggested for semen collection in order to avoid the deleterious effect of urine contamination, and for the purpose of short-term storage between 0 and 15 °C. Oxygen atmosphere is not suitable for turbot sperm storage. Turbot spermatozoa undergo cryopreservation with a high rate of success especially in a sucrose solution with 10% dimethyl sulfoxide (DMSO) and 10% egg yolk.
Key words: Turbot / sperm / storage / cryopreservation
© IFREMER-Gauthier-Villars, 1997
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