Influence of stocking ratios on production and stress indicators in Channa striata and Heteropneustes fossilis polyculture systems

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Table 5

Immune parameters of Channa striata and Heteropneustes fossilis under different stocking ratio treatments (mean ± SE).

Species	Parameter	Sampling day	T1 (1:1)	T2 (3:7)	T3 (7:3)
C. striata	Lysozyme activity (µg/mL)	Day 1	24.8 ± 1.3	24.5 ± 1.2	24.9 ± 1.4
		Day 14	25.1 ± 1.3^c	23.5 ± 1.2^b	22.0 ± 1.2^a
		Day 28	25.2 ± 1.4^c	22.6 ± 1.2^b	19.6 ± 1.1^a
		Day 56	25.3 ± 1.4^c	21.8 ± 1.2^b	17.5 ± 1.1^a
	Respiratory burst activity (OD 540 nm)	Day 1	0.78 ± 0.04	0.77 ± 0.04	0.78 ± 0.05
		Day 14	0.79 ± 0.05^c	0.74 ± 0.04^b	0.69 ± 0.04^a
		Day 28	0.79 ± 0.05^c	0.70 ± 0.04^b	0.60 ± 0.03^a
		Day 56	0.79 ± 0.05^c	0.66 ± 0.04^b	0.52 ± 0.03^a
	ACH50 (units/mL)	Day 1	131.8 ± 6.6	130.9 ± 6.5	132.0 ± 6.7
		Day 14	132.2 ± 6.7^c	125.3 ± 6.2^b	117.5 ± 5.9^a
		Day 28	132.4 ± 6.7^c	119.5 ± 5.8^b	106.2 ± 5.3^a
		Day 56	132.6 ± 6.8^c	114.2 ± 5.5^b	95.7 ± 4.9^a

H. fossilis	Lysozyme activity (µg/mL)	Day 1	22.3 ± 1.2	22.0 ± 1.1	22.5 ± 1.3
		Day 14	22.5 ± 1.2^c	20.6 ± 1.1^b	19.1 ± 1.1^a
		Day 28	22.6 ± 1.3^c	19.5 ± 1.1^b	16.5 ± 1.0^a
		Day 56	22.7 ± 1.3^c	18.4 ± 1.1^b	14.2 ± 0.9^a
	Respiratory burst activity (OD 540 nm)	Day 1	0.71 ± 0.04	0.70 ± 0.04	0.71 ± 0.04
		Day 14	0.72 ± 0.04^c	0.66 ± 0.04^b	0.61 ± 0.03^a
		Day 28	0.72 ± 0.04^c	0.62 ± 0.03^b	0.52 ± 0.03a
		Day 56	0.72 ± 0.04^c	0.58 ± 0.03^b	0.43 ± 0.02^a
	ACH50 (units/mL)	Day 1	124.5 ± 6.1	123.8 ± 6.0	124.9 ± 6.2
		Day 14	124.9 ± 6.1^c	117.2 ± 5.7^b	109.5 ± 5.4^a
		Day 28	125.1 ± 6.2^c	110.3 ± 5.4^b	96.2 ± 4.8^a
		Day 56	125.3 ± 6.2^c	103.6 ± 5.1^b	84.2 ± 4.3^a

Different superscripts in the same row indicate significant differences (p < 0.05).

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