HTTP_Request2_Exception Unable to connect to tcp://think-ws.ca.edps.org:85. Error: php_network_getaddresses: getaddrinfo failed: Name or service not known Development of a new multiplex-PCR tool for the simultaneous detection of the fish pathogens Vibrio alginolyticus, Vibrio anguillarum, Vibrio harveyi and Edwardsiella tarda | Aquatic Living Resources

Fig. 4

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Multiplex-PCR sensitivity test. Lane 1 – 250 bp ladder; lane 2 – mixture of different DNA (64.6 ng µl−1 for E. tarda; 89.5 ng µl−1 for V. alginolyticus; 110.8 ng µl−1 for V. anguillarum; 24 ng µl−1 for V. harveyi); lane 3 – mixture of different DNA (32.3 ng µl−1 for E. tarda; 44.8 ng µl−1 for V. alginolyticus; 55.4 ng µl−1 for V. anguillarum; 12 ng µl−1 for V. harveyi); lane 4 – mixture of different DNA (16.2 ng µl−1 for E. tarda; 22.4 ng µl−1 for V. alginolyticus; 27.7 ng µl−1 for V. anguillarum; 6 ng µl−1 for V. harveyi); lane 5 – mixture of different DNA (8.1 ng µl−1 for E. tarda; 11.2 ng µl−1 for V. alginolyticus; 13.9 ng µl−1 for V. anguillarum; 3 ng µl−1 for V. harveyi); lane 6 – mixture of different DNA (4 ng µl−1 for E. tarda; 5.6 ng µl−1 for V. alginolyticus; 6.9 ng µl−1 for V. anguillarum; 1.5 ng µl−1 for V. harveyi); lane 7 – mixture of different DNA (2 ng µl−1 for E. tarda; 2.8 ng µl−1 for V. alginolyticus; 3.5 ng µl−1 for V. anguillarum; 0.8 ng µl−1 for V. harveyi); lane 8 – mixture of different DNA (1 ng µl−1 for E. tarda; 1.4 ng µl−1 for V. alginolyticus; 1.7 ng µl−1 for V. anguillarum; 0.4 ng µl−1 for V. harveyi); lane 9 – mixture of different DNA (0.5 ng µl−1 for E. tarda; 0.7 ng µl−1 for V. alginolyticus; 0.9 ng µl−1 for V. anguillarum; 0.2 ng µl−1 for V. harveyi); lane 10 – mixture of different DNA (0.3 ng µl−1 for E. tarda; 0.3 ng µl−1 for V. alginolyticus; 0.4 ng µl−1 for V. anguillarum; 0.1 ng µl−1 for V. harveyi); lane 11 – control of the reaction, without DNA.

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