Development of a new multiplex-PCR tool for the simultaneous detection of the fish pathogens Vibrio alginolyticus, Vibrio anguillarum, Vibrio harveyi and Edwardsiella tarda

International Meeting on Marine Research 2016

Free Access

Fig. 2

Multiplex-PCR at different annealing temperatures and different concentrations of MgCl₂. Lane 1 – 250 bp ladder; lanes 2–5: 49 °C; lanes 6–9: 51 °C; lanes 10–13: 53 °C; lanes 2, 6, 10: 2 mM MgCl₂; lanes 3, 7, 11: 4 mM MgCl₂; lanes 4, 8, 12: 6 mM MgCl₂; lanes 5, 9, 13: 8 mM MgCl₂.

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