Flow cytometric determination of genome size in European seabass (Dicentrarchus labrax), gilthead seabream (Sparus aurata), thinlip mullet (Liza ramada), and European eel (Anguilla anguilla)

Free access article

Issue		Aquat. Living Resour. Volume 18, Number 1, January-March 2005


Page(s)		77 - 81
DOI		10.1051/alr:2005008

Aquat. Living Resour. 18 (2005) 77-81
DOI: 10.1051/alr:2005008

Flow cytometric determination of genome size in European seabass (Dicentrarchus labrax), gilthead seabream (Sparus aurata), thinlip mullet (Liza ramada), and European eel (Anguilla anguilla)

Stefano Peruzzi^{1, 2}, Béatrice Chatain¹ and Bruno Menu¹

¹ IFREMER, Laboratoire de Recherche Piscicole de Méditerranée, Chemin de Maguelone, 34250 Palavas-les-Flots, France
² Norwegian College of Fishery Science, University of Tromsø, 9037 Tromsø, Norway

(Received 19 December 2003; Accepted 5 July 2004)

Abstract
The nuclear DNA content in Dicentrarchus labrax, Sparus aurata, Liza ramada, and Anguilla anguilla, was measured by flow cytometric analysis. Male human leukocytes were used as internal reference cells. Nucleated fish erythrocytes were stained simultaneously to human leukocytes with a staining buffer containing propidium iodide (PI). Nuclear DNA contents of target species were estimated in relation to an assigned value of 7.0 pg DNA for male human leukocytes. The DNA content/nucleus ( $\pm$ CI) was $1.55 \pm 0.02$ pg for D. labrax, $1.90 \pm 0.03$ pg for S. aurata, $1.57 \pm 0.02$ pg for L. ramada, and $2.43 \pm 0.04$ pg for A. anguilla. Intraspecific DNA content variations ranged from 0-9% and averaged around 4% in all taxa. The results did not evidence significant differences in genome size between males and females in either D. labrax or L. ramada. / Anguilla anguilla

Key words: DNA content / Teleosts / Dicentrarchus labrax / Sparus aurata / Liza ramada

Corresponding author: stefanop@nfh.uit.no

© EDP Sciences, IFREMER, IRD 2005

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