EDP Sciences Journals List
Home Document
Subscriber Authentication Point
Free access article

Issue Aquat. Living Resour.
Volume 3, Number 2, April-June 1990
Page(s) 107 - 112
DOI 10.1051/alr:1990010

Aquat. Living Resour. 3 (1990) 107-112
DOI: 10.1051/alr:1990010

Polyploidy in the Manila clam, Ruditapes philippinarum. II. Chemical induction of tetraploid embryos

Alain Diter and Christian Dufy

IFREMER, Laboratoire de Pathologie et de Génétique des Invertébrés Marins, BP 133, 17390 La Tremblade, France

(Received 24 November 1989, Accepted 7 March 1990)

Abstract
Tetraploidy was induced in embryos of Ruditapes philippinarum by treating the eggs with cytochalasin B (CB). Two treatment times were found to be effective in inducing tetraploidy: 0 to 10 minutes after insemination (before formation of the first polar body) and 45 minutes after insemination (just before the first cleavage). Treatments, started 5 and 45 minutes after insemination, produced $64.4\pm7.8{\%}$ and $28.3\pm2.3{\%}$ tetraploid embryos, respectively, after 6 hours (on the average over 3 experiments). However, no tetraploids were detected in 4-month-old spat. Sperm entry delayed by the CB could be involved in induction of tetraploidy by early treatment.


Résumé
La tétraploïdie a été induite par traitement des oeufs par la cytochalasine B (CB). Deux moments d'application du traitement se sont révélés efficaces pour l'induction de la tétraploïdie: de 0 à 10 minutes après insémination (avant l'émission du premier globule polaire) et 45 minutes après insémination (juste avant le moment du premier clivage). Les traitements débutant 5 et 45 minutes après insémination ont produit respectivement $64,4\pm7,8~{\%}$ et $28,3\pm 2,3~{\%}$ d'embryons tétraploïdes à 6 heures (en moyenne sur 3 expériences). Aucun tétraploïde n'a été détecté dans le naissain de 4 mois. Un retard de la pénétration du sperme provoqué par la CB pourrait être impliqué dans l'induction de la tétraploïdie par traitement précoce.


Key words: Cytochalasin B / tetraploidy / bivalve / Ruditapes philippinarum

Mots clés : Cytochalasine B / tétraploïdie / bivalve / Ruditapes philippinarum




What is OpenURL?

The OpenURL standard is a protocol for transmission of metadata describing the resource that you wish to access. An OpenURL link contains article metadata and directs it to the OpenURL server of your choice. The OpenURL server can provide access to the resource and also offer complementary services (specific search engine, export of references...). The OpenURL link can be generated by different means.
  • If your librarian has set up your subscription with an OpenURL resolver, OpenURL links appear automatically on the abstract pages.
  • You can define your own OpenURL resolver with your EDPS Account. In this case your choice will be given priority over that of your library.
  • You can use an add-on for your browser (Firefox or I.E.) to display OpenURL links on a page (see http://www.openly.com/openurlref/). You should disable this module if you wish to use the OpenURL server that you or your library have defined.