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Issue Aquat. Living Resour. Volume 17, Number 3, July-September 2004 The "Erika" Oil Spill: Environmental Contamination and Effects in the Bay of Biscay Page(s) 269 - 272 DOI 10.1051/alr:2004030

Aquat. Living Resour. 17 (2004) 269-272
DOI: 10.1051/alr:2004030

Analysis of the adaptation to alkanes of the marine bacterium Marinobacter hydrocarbonoclasticus sp 17 by two dimensional gel electrophoresis

Guillaume Ballihaut, Benjamin Klein, Philippe Goulas, Robert Duran, Pierre Caumette and Régis Grimaud

Laboratoire d'Écologie Moléculaire, IBEAS Université de Pau, Av. de l'Université, BP 1155, 64013 PAU Cedex, France

(Received 21 November 2003; Accepted 17 May 2004 )

Abstract
To better understand the molecular mechanisms involved in the biodegradation of hydrocarbon compounds from the "Erika" oil-spill, we have studied the ability of Marinobacter hydrocarbonoclasticus strain sp 17 to cope with hexadecane as sole carbon and energy source. Growth kinetics of cultures shifted from acetate to hexadecane revealed the presence of a 20 hours adaptation phase. Changes in global protein expression in response to hexadecane was analyzed by two-dimensional gel electrophoresis. Of the 370 proteins detected 42 had their expression level altered in presence of hexadecane indicating that alkane adaptation may involve many cellular functions.

Key words: Alkanes / Biodegradation / Proteomic / 2D Electrophoresis

Corresponding author: regis.grimaud@univ-pau.fr

© EDP Sciences, IFREMER, IRD 2004

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