Long-term effects of the cryopreservation of turbot (Psetta maxima) spermatozoa
IFREMER, Laboratoire de Physiologie des Poissons, Centre de Brest, BP70, 29280 Plouzané, France
2 Muséum National d'Histoire Naturelle, Laboratoire d'Ichtyologie, 43 rue Cuvier, 75231 Paris, France
Accepted: 15 January 1998
The survival of turbot eggs and the rearing capacities of larvae stemmed from artificial fertilization practices using frozen-thawed spermatozoa were evaluated. Furthermore, the viability of sperm samples stored during a 9 month period in liquid nitrogen was assessed. No significant difference in the fertilization rate, hatching rate, survival and wet weight of 10-day old larvae were observed using fresh or frozen-thawed spermatozoa. The motility recorded at 10 s and 60 s post-activation and the fertilization capacity of frozen-thawed spermatozoa were not significantly decreased during a 9 month storage period in liquid nitrogen. These results confirm the high quality of the turbot spermatozoa stemmed from the cryopreservation process, allowing their use for routine aquaculture practices.
Key words: turbot / Psetta maxima / sperm / cryopreservation / hatching
© Elsevier, IRD, Inra, Ifremer, Cemagref, CNRS, 1998